Centrifugation is a method in which solid or liquid particles of different densities are separated by rotating them in the tube in a horizontal circle. The heavier suspended particles move down and lighter particles remain on top. In this method, the mixture is rotated at a high speed in a test tube in a machine called centrifuge.
Chromatography is a technique of separating the constituents of a mixture of liquids. It is also used for gases. These substances should be such that they do not go undergo a chemical reaction with each other. The substances on which the mixtures are absorbed are called absorbents. Some of them are silica gel, filter paper, cellulose powder, alumina, etc.
Paper chromatography proceeds by a mechanism which is partly partition and partly absorption. The solute mixture, generally dissolved in water, is placed on a strip chromatographic paper and a second solvent is allowed to travel along the strip. It will extra the solute as a result distribution of the solvent between the two solvents.
Application of Chromatography
This method of separation is used to separate various chemicals in chemistry. Moreover, it is used in following sectors:
What are the advantages of using chromatography?
The advantages of chromatography are:
If two different types of particles have the same speed in a medium is chromatography possible? Write with a reason.
No, because they are absorbed to a same extent and can’t be separated from each other.
What is chromatography? Write is short about absorbent chromatography.
Chromatography is a technique that is used for separation the component of a mixture by passing it through a column or sheet of an absorbing material.
Absorbent chromatography is based on the principle that "a certain absorbent can absorb different substances to different extents” When a solution containing two or more than two dissolved substances is to be separated, it is allowed to pour into a vertical glass tube packed with finely divided absorbent like alumina. The colors of dissolved substances in a solution are separated in the form of bands.
What is centrifuging? How is it done?
It is the process of separation very fine suspended solid particles or semi-solid from a mixture by rotating the mixture kept in a centrifuging machine at high speed.
It is done by rotating a machine at a high speed.
Write the differences between filtration and chromatography.
|1||This method is used to remove an insoluble substance from a mixture by the use of filter paper is called filtration.||Chromatography is the method of separation of colours / pigments from a solution|
|2||It can separate insoluble substance from the mixture.||This process is used to separate colour of mixture.|
Write the applications of chromatography.
The applications of chromatography are:
Write short note on paper chromatography.
Paper chromatography proceeds by a mechanism which is partly partition aand partly absorption. The solute mixture, generally dissolved in water, is placed on a strip chromatographic paper and a second solvent is allowed to travel along the strip. It will extract the solute as a result distribution of the solvent between the two solvents.
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